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課程名稱 |
呼吸毒理學概論
Fundamentals of Inhalation Toxicology |
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開課學期 |
100-2 |
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授課對象 |
公共衛生學院 職業醫學與工業衛生研究所 |
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授課教師 |
鄭尊仁 |
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課號 |
OMIH7038 |
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課程識別碼 |
841 M1690 |
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班次 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期三5,6(12:20~14:10) |
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上課地點 |
公衛210 |
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備註 |
總人數上限：25人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1002INHYOX |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
本課程對象為公衛學院環衛所與職衛所及其他相關系所對呼吸毒理有興趣之碩士及博士班研究生。上課方式包括講演與討論及實驗課程，內容包括呼吸暴露系統的建立與監測、呼吸道生物學、呼吸道對吸入物質的毒理反應等。 |
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課程目標 |
使學生瞭解呼吸毒理基本原理，作為呼吸毒理研究的基礎。 |
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課程要求 |
課前閱讀指定教材及上課參與討論。 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
呼吸毒理學課程大綱
教科書："Inhalation Studies: Foundations and Techniques", Second Edition, Edited by
Robert F. Phalen. Informa Healthcare, 2008
1.簡介
Michael A. Dorato. 1990.Overview of Inhalation Toxicology. Environmental Health Perspectives Vol. 85, pp. 163-170.
2. 微粒產生及監測
Robert F. Phalen,2008.Ch3,4
呼吸毒理應用
3. 奈米微粒暴露系統毒理研究
討論：台灣（李曜全）韓國及日本的奈米產生器論文報告及比較
(1) Manabu Shimada. 2009.Development and Evaluation of an Aerosol Generation
and Supplying System for Inhalation Experiments of Manufactured Nanoparticles. Environ. Sci. Technol 43, 5529–5534
(2) 李曜全.可控制奈米微粒產生技術開發.2008.勞工安全衛生研究季刊第17卷第2期第153-162頁
(3) Jun Ho Ji et al.2007.Long-Term Stability Characteristics of Metal Nanoparticle Generator Using Small Ceramic Heater for Inhalation Toxicity Studies. Inhalation Toxicology, 19:745-751
(4) Philip Demokritou et al.2010. Development and characterization of a Versatile Engineered Nanomaterial Generation System (VENGES) suitable for toxicological studies. Inhalation Toxicology,22(S2):107-116.
(5) Yasuo Morimoto et al.2010. Inflammogenic effect of well-characterized fullerenes in inhalation and intratracheal instillation studies. Particle and Fibre Toxicology, 7:4
(6) Jun Ho Ji et al.2007.Twenty-Eight-Day Inhalation Toxicity Study of Silver Nanoparticles in Sprague-Dawley Rats. Inhalation Toxicology, 19:857–871.
(7) Jae Hyuck Sung et al. 2009. Subchronic Inhalation Toxicity of Silver Nanoparticles. Toxicological Sciences, 108(2),452-461
(8) Gi Soo Kang et al. 2011. Long-term inhalation exposure to nickel nanoparticles exacerbated atherosclerosis in a susceptible mouse model. Environment Health Perspectives, 119(2):176-181
4. 奈米銀暴露及毒理
(1) Jae Hyuck Sung et al. 2008. Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles. Inhal Toxicol;20: 567–74
(2) Jae Hyuck Sung et al. 2010. Acute inhalation toxicity of silver nanoparticles . Toxicology and Industrial Health; 27(2) 149–154
5. 實驗：奈米銀產生及監測
6. 氣體產生及監測
討論：比較微粒及氣體產生及監測之異同
Phalen,Ch3,4
暴露系統
討論：比較全身及鼻部暴露之優缺點，參觀暴露腔
Phalen, Ch5
7. 溫書假
8. 期中考
9. 呼吸道結構與功能（包括沈積清除等防衛機轉）
討論：比較人類與動物結構之異同
Phalen.Ch2
10. 毒性測試
Phalen.Ch6
11.大氣微粒濃縮及毒理研究
討論：大氣微粒濃縮的原理及毒性研究
(1) Constantinos Sioutas et al. 1995. A technique to expose animals to concentrated fine ambient aerosols. Environment Health Perspectives, 103:172-177
(2) Polina Maciejczyk, Mianhua Zhong, Qian Li, Judy Xiong,Christine Nadziejko, and Lung Chi Chen. 2005Effects of Subchronic Exposures to Concentrated Ambient Particles (CAPs) in Mice: II. The Design of a CAPs Exposure System for Biometric Telemetry Monitoring. Inhalation Toxicology, 17:189–197
(3) Qinghua Sun et al.2005. Long-term Air Pollution Exposure and Acceleration of Atherosclerosis and Vascular Inflammation in an Animal Model. JAMA, 294(23):3003-3010
(4) Cheng TJ et al. 2003. Effects of concentrated ambient particles on heart rate and blood pressure in pulmonary hypertensive rats. Environ Health Perspect, 111(2):147-150
(5) Lei YC et al. 2004. Effects of concentrated ambient particles on airway responsiveness and pulmonary inflammation in pulmonary hypertensive rats. Inhalation Toxicology, 16(11-12):785-792
(6) Chang CC et al. 2004. Effects of Concentrated Ambient Particles on Heart Rate, Blood Pressure, and Cardiac Contractility in Spontaneously Hypertensive Rats. Inhalation Toxicology, 16(6-7):421-429
12.呼吸毒理實驗室設備
13. 氣管灌注及毒性研究；動物實驗倫理(動物實驗準則)
(1) Society of Toxicology . 1999.Guiding Principles in the Use of Animals in Toxicology
討論：比較呼吸及氣管灌注之優缺點
(2) Joseph D Brain et al.1975. Pulmonary distribution of particles given by
intratracheal instillation or by aerosol inhalation. Environmental Research
11,13-33
(3) B K J. Leong et al.1998. Quantitative morphometric analysis of pulmonary
deposition of aerosol particles inhaled via intratracheal nebulization, intratracheal
instillation or nose-only inhalation in rats. J. Appl. Toxicol. 18, 149–160
(4) Yasuo Oka et al. 2006.A reliable method for Intratracheal Instillation of Materials
to the Entire Lung in Rats. J Toxicol Pathol. 19:107-109.
(5) Yasuo Morimoto et al.2010. Inflammogenic effect of well-characterized fullerenes in inhalation and intratracheal instillation studies. Particle and Fibre Toxicology, 7:4
(6) Warheit DB et al. 2009. Nanoscale and Fine Zinc Oxide Particles :Can in Vitro Assays Accurately Forecast Lung Hazards following Inhalation Exposures? Environmental Science and Technology, 43(20):7939-7945
(7) Chang CC et al. 2007. Interaction Effects of Ultrafine Carbon Black with Iron and Nickel on Heart Rate Variability in Spontaneously Hypertensive Rats. Environ Health Perspect, 115(7):1012-1017
(8) Yan YH et al. 2008. Effects of Diesel Exhaust Particles on Left Ventricular Function in Isoproterenol-Induced Myocardial Injury and Healthy Rats. Inhalation Toxicology, 20(2):199-203
(9) Lei YC et al. 2005. Enhanced oxidative stress and endothelial dysfunction in streptozotocin-diabetic rats exposed to fine particles. Environ Res, 99(3):335-343
14.實驗：氣管灌注及呼吸道灌洗
15.實驗：氣管灌注及呼吸道灌洗
16.實驗：肺部功能
17.口頭報告
18.期末考週
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參考書目 |
a. ”Concepts in Inhalation Toxicology.” Edited by R.O McClellan and R.F
Henderson 2nd edition.1995, Taylor and Francis, Washington, DC
b. "Methods in Inhalation Toxicology", Edited by
Robert F. Phalen. CRC Press, 1997 |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
上課參與及報告 |
20% |
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2. |
期中考 |
40% |
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3. |
期末口頭及書面報告 |
40% |
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週次 |
日期 |
單元主題 |
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第1週 |
02/22 |
Introduction
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第2週 |
02/29 |
Generation and characterization of aerosols (Ch 3,4) |
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第3週 |
03/07 |
Nanoparticles Generation 討論:台灣、韓國及日本的奈米產生器論文報告及比較 |
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第4週 |
03/14 |
Laboratory (nanoparticles generation ) |
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第5週 |
03/21 |
Laboratory (nanoparticles generation ) |
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第6週 |
03/28 |
Generation and monitoring of gas (Ch 3,4) 討論:比較微粒及氣體產生及監測之異同
Methods for Exposing Subjects (Ch 5) 討論:比較全身及鼻部暴露之優缺點
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第7週 |
04/04 |
溫書假 |
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第8週 |
04/11 |
Midterm |
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第9週 |
04/18 |
Morphology of the respiratory tract and function (Ch2) 討論:比較人類與動物結構之異同 |
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第10週 |
04/25 |
Toxicity testing (Ch6) |
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第11週 |
05/02 |
Specific exposure methodologies 討論：大氣微粒濃縮的原理及毒性研究 |
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第12週 |
05/09 |
Inhalation toxicology laboratory and review |
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第13週 |
05/16 |
A. Ethics of animal research Laboratory and Safety;
B. Intratracheal instillation and toxicity
討論:比較呼吸及氣管灌注之優缺點
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第14週 |
05/23 |
Laboratory (intratracheal instillation and lavage) |
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第15週 |
05/30 |
Laboratory (intratracheal instillation and lavage) |
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第16週 |
06/06 |
Laboratory (lung function) |
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第17週 |
06/13 |
Oral presentation |
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第18週 |
06/20 |
Final |
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